MAB3611

antibody from R&D Systems

Targeting: CCL19 CKb11, ELC, exodus-3, MIP-3b, SCYA19

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3611

Provider

R&D Systems

Product name

Human CCL19/MIP-3 beta Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human CCL19/MIP-3 beta in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q99731


Isotype

IgG

Antibody clone number

948918

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CCL19/MIP-3 beta and Neutrali-zation by Human CCL19/MIP-3 beta Antibody. Recombinant Human CCL19/MIP-3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Antibody (Catalog # MAB3611). The ND₅₀ is typically 0.1-0.5 µg/mL.