AF361

antibody from R&D Systems

Targeting: CCL19 CKb11, ELC, exodus-3, MIP-3b, SCYA19

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF361

Provider

R&D Systems

Product name

Human CCL19/MIP-3 beta Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse (rm) MIP-3 beta , recombinant human (rh) GRO alpha , rhHCC-4, rhEotaxin, rmMIP-3 alpha , recombinant rat MIP-3 alpha , and rhMIP-3 alpha is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q99731


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Age-Related Changes to Human Tear Composition.

Micera A, Di Zazzo A, Esposito G, Longo R, Foulsham W, Sacco R, Sgrulletta R, Bonini S
Investigative ophthalmology & visual science 2018 Apr 1;59(5):2024-2031

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Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus.

Connelly KL, Kandane-Rathnayake R, Hoi A, Nikpour M, Morand EF
Scientific reports 2016 Jul 25;6:29909

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Expression of podoplanin and other lymphatic markers in the human anterior eye segment.

Birke K, Lütjen-Drecoll E, Kerjaschki D, Birke MT
Investigative ophthalmology & visual science 2010 Jan;51(1):344-54

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CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.

Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
European journal of immunology 2008 Nov;38(11):3050-9

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Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling.

Shields JD, Fleury ME, Yong C, Tomei AA, Randolph GJ, Swartz MA
Cancer cell 2007 Jun;11(6):526-38

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CCL19/MIP-3 beta by Western Blot. Western blot shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CCL19/MIP-3 beta by Simple Western^TM. Simple Western lane view shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. A specific band was detected for CCL19/MIP-3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CCL19/MIP-3 beta and Neutrali-zation by Human CCL19/MIP-3 beta Antibody. Re-combinant Human CCL19/MIP-3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND₅₀ is typically 2-10 µg/mL.