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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP6245a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP6245a, RRID:AB_2188921
- Product name
- SIRT6 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Direct evidence of sirtuin downregulation in the liver of non-alcoholic fatty liver disease patients.
Wu T, Liu YH, Fu YC, Liu XM, Zhou XH
Annals of clinical and laboratory science 2014 Fall;44(4):410-8
Annals of clinical and laboratory science 2014 Fall;44(4):410-8
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of SIRT6 Antibody (C-term) (Cat.#AP6245a) in mouse stomach tissue lysates (35ug/lane). SIRT6 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
