Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- F54008 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SIRT6 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific SIRT6 antibody is suitable for use in Western blot/Immunocytochemistry/Immunofluorescence/Immunoprecipitation applications with human, mouse and rat, primate samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 6C9-D10-D3
- Vial size
- 0.1 ml
- Concentration
- In PBS with 50% glycerol and 0.03% ProClin 300
- Storage
- Store the SIRT6 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Jurkat, MCF7, HCT116, K562, monkey COS7, mouse C2C12, mouse NIH3T3 and human HeLa cell lysates using SIRT6 antibody at 1:500. Predicted molecular weight ~42 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of HeLa cells, fixed by 4% paraformaldehyde, with SIRT6 antibody at 1:100.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of SIRT6 from HeLa cell lysate using SIRT6 antibody. The precipitate was subsequently immunoblotted with the same mAb.