Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
Reference
Comment
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- Product number
- STJ99097 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-SIRT6 antibody [6C9-D10-D3] (STJ99097)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Nad-Dependent Protein Deacetylase Sirtuin-6 is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunoprecipitation research applications.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Jurkat, MCF7, HCT116, K562, COS7, C2C12, 3T3 and Hela cell lysates using SIRT6 mouse mAb (1:500 diluted).Predicted band size:42, 36KDa.Observed band size:42, 36KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using SIRT6 mouse mAb.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells fixed fixed by 4% paraformaldehyde and using SIRT6 mouse mAb (dilution 1:100).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA