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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-85654 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85654, RRID:AB_11012633
- Product name
- Rabbit Polyclonal UBE2J1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human UBE2J1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2J1 Antibody [NBP1-85654] - Analysis in control (vector only transfected HEK293T lysate) and UBE2J1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2J1 Antibody [NBP1-85654] - Staining in human lymph node and skeletal muscle tissues using anti-UBE2J1 antibody. Corresponding UBE2J1 RNA-seq data are presented for the same tissues.
