55260-1-AP

antibody from Proteintech Group

Targeting: VDAC3 HD-VDAC3

Provider product page for 55260-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [28]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 55260-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#55260-1-AP, RRID:AB_10973676
Product name: VDAC3 antibody
Antibody type: Polyclonal
Description: VDAC3 antibody (Cat. #55260-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

VDAC3 protein structure - 55260-1-AP shown in red.

Submitted references STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria.

Orantos-Aguilera Y, Sanchez-Lopez I, Pascual-Caro C, Gómez-Suaga P, Area-Gomez E, Pozo-Guisado E, Montesinos J, Martin-Romero FJ
The EMBO journal 2026 Mar;45(5):1760-1793

Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer.

Yang F, Wang M, Hu S, Guan X, Zhao K, Zhou Y, Yao H, Zhang T, Li L, Gao Y, Zhao S, Liu N, Xiao W, Xiang Y, Miao H
Signal transduction and targeted therapy 2026 Mar 26;11(1)

Methodological approaches for dissecting ferroptosis in lens epithelial cells and its role in diabetic cataract progression.

Xu H, Wang W, Li J, Zhao X, Zhang H
Methods (San Diego, Calif.) 2026 Jun;250:14-24

Therapeutic potential of magnesium diboride nanosheets in PAH-associated right heart failure: Integrated multi-omics analysis reveals ferroptosis suppression via LC3/ATG5/NCOA4/FTH1 pathway.

Jiao Q, Yang Y, Xu X, Hao J, Guo J, Wu G, Pang S, Yang W, Zhao Y, Wang L, Wang H, Dong P, Wang Y, Wang T
Free radical biology & medicine 2026 Feb 16;244:1-16

Tartrate-Resistant Acid Phosphatase 5 (TRAP/ACP5) aggravates atherosclerosis by regulating macrophage polarization and promoting ferroptosis.

Ma X, Li Z, Liu H, Wang M, Gao F, Xiao Z, Qi M, Liu Z, Li Z, Huang J, Jiang Z, Feng Q, Wei D, Liu Z, Wang Z, Xiao S
Free radical biology & medicine 2026 Apr;247:486-501

Integrated Multilayer Omics Reveals the Underlying Mechanisms in Xylazine-Related Heart Injury in Rats.

Ou Y, Mai T, Wang N, Li Z, Chen Y, Zhao S
International journal of molecular sciences 2025 Sep 2;26(17)

Integration of transcriptomics and metabolomics to reveal crizotinib-induced liver injury in mice.

Chen H, Liu H, Wei J, Liu R, Tian X
European journal of pharmacology 2025 Nov 5;1006:178096

Cleavage of CAD by caspase-3 determines the cancer cell fate during chemotherapy.

Ma J, Zhao J, Zhang C, Tan J, Cheng A, Niu Z, Lin Z, Pan G, Chen C, Ding Y, Zhong M, Zhuang Y, Xiong Y, Zhou H, Zhou S, Xu M, Ye W, Li F, Song Y, Wang Z, Hong X
Nature communications 2025 May 30;16(1):5006

Gut microbiota-derived glutathione from metformin treatment alleviates intestinal ferroptosis induced by ischemia/reperfusion.

Wang F, Wang X, Wang C, Yan W, Xu J, Song Z, Su M, Zeng J, Han Q, Ruan G, Zhang E, Wang W
BMC medicine 2025 May 13;23(1):285

Targeting YBX1-m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma.

Li O, An K, Wang H, Li X, Wang Y, Huang L, Du Y, Qin N, Dong J, Wei J, Sun R, Shi Y, Guo Y, Sun X, Yang Y, Yang YG, Kan Q, Tian X
Clinical and translational medicine 2025 Mar;15(3):e70270

Mitochondrial fumarate inhibits Parkin-mediated mitophagy.

Ham SJ, Bang S, Woo D, Jo JY, Yoo T, Yoon E, Kyoung Y, Baek D, Kim JS, Chung J
Molecular cell 2025 Jun 19;85(12):2287-2302.e9

TP53INP2 promotes mitophagic degradation of YAP to impede dedifferentiated liposarcoma development.

Wang Y, Huang Y, Wang L, Chen Z, Zhou L, Xiang F, Li G, Yang J, Chen R, Xu Q, Shen Y
Oncogene 2025 Jul;44(25):2054-2063

Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury.

Zhang L, Yang S, Cui H, Hang C, Wang X, An L, Shan Z, Liang Z, Shao R, Tang Z
Scientific reports 2025 Feb 7;15(1):4607

Proteomics reveals the pharmacological mechanism of flavonoids from Astragali Complanati Semen in preventing chronic liver injury.

Chen L, Kong X, Zhou R, Hu J, Zhou R, Song Z, Tang Z, Wang M
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Oct;133:155910

Lysosomal iron accumulation and subsequent lysosomes-mitochondria iron transmission mediate PFOS-induced hepatocyte ferroptosis.

Li J, Feng R, Yang W, Liang P, Qiu T, Zhang J, Sun X, Li Q, Yang G, Yao X
Ecotoxicology and environmental safety 2024 Oct 1;284:116890

Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia.

Tang Y, Wu J, Sun X, Tan S, Li W, Yin S, Liu L, Chen Y, Liu Y, Tan Q, Jiang Y, Yang W, Huang W, Weng C, Wu Q, Lu Y, Yuan H, Xiao Q, Chen AF, Xu Q, Billiar TR, Cai J
Cell reports 2024 May 28;43(5):114237

ACSL4-mediated ZIP7-VDAC3 interaction regulates endoplasmic reticulum-mitochondria iron transfer in hepatocytes under PFOS exposure.

Liang P, Tian K, Yang W, Feng R, Li Y, Hu L, Wang K, Qiu T, Zhang J, Sun X, Yao X
The Science of the total environment 2024 Dec 20;957:177679

BRCA1-Mediated Dual Regulation of Ferroptosis Exposes a Vulnerability to GPX4 and PARP Co-Inhibition in BRCA1-Deficient Cancers.

Lei G, Mao C, Horbath AD, Yan Y, Cai S, Yao J, Jiang Y, Sun M, Liu X, Cheng J, Xu Z, Lee H, Li Q, Lu Z, Zhuang L, Chen MK, Alapati A, Yap TA, Hung MC, You MJ, Piwnica-Worms H, Gan B
Cancer discovery 2024 Aug 2;14(8):1476-1495

G6PD maintains the VSMC synthetic phenotype and accelerates vascular neointimal hyperplasia by inhibiting the VDAC1-Bax-mediated mitochondrial apoptosis pathway.

Zhang T, Cao RJ, Niu JL, Chen ZH, Mu SQ, Cao T, Pang JX, Dong LH
Cellular & molecular biology letters 2024 Apr 8;29(1):47

Longitudinal diffusion barriers imposed by myofilaments and mitochondria in murine cardiac myocytes.

Deisl C, Chung JH, Hilgemann DW
The Journal of general physiology 2023 Oct 2;155(10)

De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence.

Yang C, Zhao Y, Wang L, Guo Z, Ma L, Yang R, Wu Y, Li X, Niu J, Chu Q, Fu Y, Li B
Nature cell biology 2023 Jun;25(6):836-847

The genomic signature of resistance to platinum-containing neoadjuvant therapy based on single-cell data.

Sui Q, Hu Z, Jin X, Bian Y, Liang J, Zhang H, Yang H, Lin Z, Wang Q, Zhan C, Chen Z
Cell & bioscience 2023 Jun 8;13(1):103

Prevotella histicola suppresses ferroptosis to mitigate ethanol-induced gastric mucosal lesions in mice.

Wang S, Wu D, Wu F, Sun H, Wang X, Meng H, Lin Q, Jin K, Wang F
BMC complementary medicine and therapies 2023 Apr 14;23(1):118

The lncRNA BDNF-AS/WDR5/FBXW7 axis mediates ferroptosis in gastric cancer peritoneal metastasis by regulating VDAC3 ubiquitination.

Huang G, Xiang Z, Wu H, He Q, Dou R, Lin Z, Yang C, Huang S, Song J, Di Z, Wang S, Xiong B
International journal of biological sciences 2022;18(4):1415-1433

Proteomic Studies of the Mechanism of Cytotoxicity, Induced by Palytoxin on HaCaT Cells.

Cheng D, Deng B, Tong Q, Gao S, Xiao B, Zhu M, Ren Z, Wang L, Sun M
Toxins 2022 Apr 10;14(4)

Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia.

Zhu T, Liu B, Wu D, Xu G, Fan Y
Frontiers in cell and developmental biology 2021;9:740884

Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress.

Yang M, Sun J, Stowe DF, Tajkhorshid E, Kwok WM, Camara AKS
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2020 Sep 9;54(5):853-874

VDAC2 enables BAX to mediate apoptosis and limit tumor development.

Chin HS, Li MX, Tan IKL, Ninnis RL, Reljic B, Scicluna K, Dagley LF, Sandow JJ, Kelly GL, Samson AL, Chappaz S, Khaw SL, Chang C, Morokoff A, Brinkmann K, Webb A, Hockings C, Hall CM, Kueh AJ, Ryan MT, Kluck RM, Bouillet P, Herold MJ, Gray DHD, Huang DCS, van Delft MF, Dewson G
Nature communications 2018 Nov 26;9(1):4976

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse testis tissue were subjected to SDS PAGE followed by western blot with 55260-1-AP(VDAC3 antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human testis using 55260-1-AP(VDAC3 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The VDAC3 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human VDAC3. This antibody recognizes human, mouse, rat antigen. The VDAC3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 55260-1-AP.pdf

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