IC6166A

antibody from Novus Biologicals

Targeting: EOMES TBR2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC6166A

Provider

Novus Biologicals

Product name

Mouse Monoclonal EOMES Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human EOMES in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Hobit expression by a subset of human liver-resident CD56^bright Natural Killer cells.

Lunemann S, Martrus G, Goebels H, Kautz T, Langeneckert A, Salzberger W, Koch M, J Bunders M, Nashan B, van Gisbergen KPJM, Altfeld M
Scientific reports 2017 Jul 27;7(1):6676

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of EOMES in Mesendoderm-differentiated BG01V Human Cells by Flow Cytometry. Mesendoderm-differentiated BG01V human embryonic stem cells were stained with Mouse Anti-Human EOMES APC-conjugated Monoclonal Antibody (Catalog # IC6166A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.