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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- MA5-24291 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EOMES Monoclonal Antibody (644730)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 644730
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Zika Virus Infects Intermediate Progenitor Cells and Post-mitotic Committed Neurons in Human Fetal Brain Tissues.
Lin MY, Wang YL, Wu WL, Wolseley V, Tsai MT, Radic V, Thornton ME, Grubbs BH, Chow RH, Huang IC
Scientific reports 2017 Nov 1;7(1):14883
Scientific reports 2017 Nov 1;7(1):14883
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of EOMES in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm. Samples were incubated in EOMES monoclonal antibody (Product # MA5-24291) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of BG01V human embryonic stem cells differentiated to mesendoderm were stained with mouse Anti-human EOMES Monoclonal Antibody (Product # MA5-24291) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of EOMES in BG01V human embryonic stem cells differentiated to mesendoderm. Samples were incubated in EOMES monoclonal antibody (Product # MA5-24291) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
