Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-47818 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EOMES Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of human EOMES Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47818) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using human EOMES Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47818) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-human EOMES Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47818) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.