14-4876-80

antibody from Invitrogen Antibodies

Targeting: EOMES TBR2

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-4876-80

Provider

Invitrogen Antibodies

Product name

EOMES Monoclonal Antibody (21Mags8), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This 21Mags8 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Antibody clone number

21Mags8

Concentration

0.5 mg/mL

References

Submitted references

T-bet is a key modulator of IL-23-driven pathogenic CD4(+) T cell responses in the intestine.

Krausgruber T, Schiering C, Adelmann K, Harrison OJ, Chomka A, Pearson C, Ahern PP, Shale M, Oukka M, Powrie F
Nature communications 2016 May 19;7:11627

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TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Weidgang CE, Russell R, Tata PR, Kühl SJ, Illing A, Müller M, Lin Q, Brunner C, Boeckers TM, Bauer K, Kartikasari AE, Guo Y, Radenz M, Bernemann C, Weiß M, Seufferlein T, Zenke M, Iacovino M, Kyba M, Schöler HR, Kühl M, Liebau S, Kleger A
Stem cell reports 2013;1(3):248-65

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Unique features of naive CD8+ T cell activation by IL-2.

Cho JH, Kim HO, Kim KS, Yang DH, Surh CD, Sprent J
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5559-73

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The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation.

Costello I, Pimeisl IM, Dräger S, Bikoff EK, Robertson EJ, Arnold SJ
Nature cell biology 2011 Aug 7;13(9):1084-91

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Anomalous type 17 response to viral infection by CD8+ T cells lacking T-bet and eomesodermin.

Intlekofer AM, Banerjee A, Takemoto N, Gordon SM, Dejong CS, Shin H, Hunter CA, Wherry EJ, Lindsten T, Reiner SL
Science (New York, N.Y.) 2008 Jul 18;321(5887):408-11

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen cell lysate were resolved by SDS-PAGE and electroblotted onto a PVDF membrane. The membrane was probed with 2 µg/mL of Anti-Human/Mouse EOMES Purified and revealed using an Anti-Rat IgG HRP antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Eomes directly binds conserved T-box sites within the Mesp1 locus to activate expression. (a) Mesp1 robustly expressed in wild-type embryos at E7.0, is absent in Eomes N/CA ;Sox2Cre mutants. At slightly later stages (E7.25) Eomes N/CA ;Sox2Cre embryos occasionally show weak expression that likely reflects activity of Tbx6 , known to be expressed in E7.5 Eomes mutants 1 . (b) As judged by qRT-PCR Mesp1 and Mesp2 transcripts are dramatically reduced in E7.25 Eomes mutant embryos. Error bars represent standard deviation (s.d.), n= 5 per genotype. (c) Diagrammatic representation of cis -regulatory elements in the Mesp1/2 locus. The positions of previously identified T-box sites within the Mesp1/2 EME and Mesp2 PSME (nomenclature according to ref 26 , 27 ) and a novel putative T-box site identified near the Mesp1 TSS are indicated. The Mesp2 PSME contains 3 binding elements (Sites B, G, D) that contain T-box binding motifs. T-box consensus sequences are indicated in red. Red bars indicate areas amplified by qPCR after ChIP using different antibodies. Ex1 , Exon1; Ex2 , Exon2; EME , early mesoderm enhancer; PSME , pre-somitic mesoderm enhancer; TSS , transcriptional start site; Tbx , T-box site. (d) ChIP analysis of P19Cl6 cells treated for 4 days with DMSO using antibodies specific for Eomes, RNA-Polymerase II (PolII) or an IgG control. Specific enrichment for genomic loci containing T-box sites ( Mesp1_ TSS, Mesp1 _Tbx, Mesp2 _Tbx) was observed using the Eomes-specific a

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 T-bet-deficient T cells are hyper-responsive to IL-23. C57BL/6 Rag1 -/- mice were injected i.p. with either WT or Tbx21 -/- CD4 + CD25 - CD45RB hi T cells or a 1:1 mixtures of WT (CD45.1 + ) and Tbx21 -/- (CD45.2 + ) CD4 + CD25 - CD45RB hi T cells. Mice were killed when recipients of Tbx21 -/- T cells developed clinical signs of disease (~5 weeks) and assessed for intestinal inflammation. ( a ) Colitis scores. ( b ) Frequencies of IL-17A + and/or IFN-gamma + cells in the colon of mice receiving mix of WT and Tbx21 -/- CD4 + T cells. ( c ) CD4 + T cells were purified by flow cytometry from the inflamed colon of mice receiving a mix of WT and Tbx21 -/- CD4 + T cells and mRNA levels of indicated genes were analysed by quantitative reverse transcription PCR (qRT-PCR). ( d ) ChIP-qRT-PCR for the occupancy of H3K4me3 or RNA Pol II around the TSS of indicated genes in CD4 + T cells purified as described in c . ( e ) Immunoblot for pY705-STAT3, total STAT3, EOMES, Runx3 and Actin on whole cells lysates of CD4 + T cells purified as in c , left unstimulated or stimulated with IL-23 for 12 h. ( f ) Concentrations of indicated cytokines secreted into culture medium by CD4 + T cells purified and stimulated as described in e . Data in a - c and f represent results from one of two independent experiments ( n =5 for WT, n =6 for Tbx21 -/- , n =6 for WT+ Tbx21 -/- ). Bars are the mean and each symbol represents an individual mouse. Bars are the mean and error bars represent s.e.m. Da