Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB3272 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3272, RRID:AB_1574600
- Product name
- EIF2AK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EIF2AK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Autophosphorylation of threonine 485 in the activation loop is essential for attaining eIF2alpha kinase activity of HRI.
Regulation of protein synthesis by heme-regulated eIF-2 alpha kinase.
Rafie-Kolpin M, Han AP, Chen JJ
Biochemistry 2003 Jun 3;42(21):6536-44
Biochemistry 2003 Jun 3;42(21):6536-44
Regulation of protein synthesis by heme-regulated eIF-2 alpha kinase.
Chen JJ, London IM
Trends in biochemical sciences 1995 Mar;20(3):105-8
Trends in biochemical sciences 1995 Mar;20(3):105-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EIF2AK1 polyclonal antibody (Cat # PAB3272) in A-375 cell lysate. EIF2AK1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with EIF2AK1 polyclonal antibody (Cat # PAB3272) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)