MAB33051

antibody from R&D Systems

Targeting: F7

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB33051

Provider

R&D Systems

Product name

Mouse Coagulation Factor VII Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse Coagulation Factor VII in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Factor X, rhFactor XI, rhPROC, rhThrombin, rhKallikrein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or Kallikrein B1 is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P70375


Isotype

IgG

Antibody clone number

406707

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Coagulation Factor VII in Mouse Liver. Coagulation Factor VII was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse Coagulation Factor VII Monoclonal Antibody (Catalog # MAB33051) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.