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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22059 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Factor X/Xa Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: rat liver, human F10-transfected 293T cells. Predicted reactivity: Rhesus Monkey (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Factor X was performed by separating 30 µg of HepG2 conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Factor X Polyclonal Antibody (Product # PA5-22059) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Factor X was performed by separating 30 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Factor X Polyclonal Antibody (Product # PA5-22059) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Factor X/Xa Polyclonal Antibody detects F10 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human F10-transfected 293T cells.10 % SDS-PAGE. Factor X/Xa Polyclonal Antibody (Product # PA5-22059) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Factor X in methanol-fixed HeLa cells using a Factor X polyclonal antibody (Product # PA5-22059) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Factor X antibody (Product # PA5-22059) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Factor X (Product # PA5-22059) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
