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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29118 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Factor X/Xa Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, human F10-transfected 293T cells, GL261, C8D30.
- Concentration
- 1.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Factor X using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Factor X polyclonal antibody (Product # PA5-29118) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Factor X was performed by separating 30 µg of HepG2 whole cell extract and conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Factor X Polyclonal Antibody (Product # PA5-29118) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Factor X/Xa Polyclonal Antibody detects F10 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human F10-transfected 293T cells.10% SDS-PAGE. Factor X/Xa Polyclonal Antibody (Product # PA5-29118) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Factor X/Xa Polyclonal Antibody detects F10 protein by western blot analysis. A. 30 µg GL261 whole lysate/extract. B. 30 µg C8D30 whole cell lysate/extract.10% SDS-PAGE. Factor X/Xa Polyclonal Antibody (Product # PA5-29118) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Factor X in methanol-fixed HeLa cells using a Factor X polyclonal antibody (Product # PA5-29118) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Factor X (Product # PA5-29118) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
