Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 600-401-938 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-938, RRID:AB_828456
- Product name
- Anti-Myosin (RABBIT) Antibody - 600-401-938
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells.
Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells.
Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function.
Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration.
Aguilar-Cuenca R, Llorente-González C, Chapman JR, Talayero VC, Garrido-Casado M, Delgado-Arévalo C, Millán-Salanova M, Shabanowitz J, Hunt DF, Sellers JR, Heissler SM, Vicente-Manzanares M
Current biology : CB 2020 Jul 6;30(13):2446-2458.e6
Current biology : CB 2020 Jul 6;30(13):2446-2458.e6
Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells.
Deng JT, Bhaidani S, Sutherland C, MacDonald JA, Walsh MP
PloS one 2019;14(12):e0226406
PloS one 2019;14(12):e0226406
Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function.
Lahey KA, Ronaghan NJ, Shang J, Dion SP, Désilets A, Leduc R, MacNaughton WK
PloS one 2017;12(7):e0180259
PloS one 2017;12(7):e0180259
Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration.
Logue JS, Cartagena-Rivera AX, Baird MA, Davidson MW, Chadwick RS, Waterman CM
eLife 2015 Jul 11;4:e08314
eLife 2015 Jul 11;4:e08314
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2). Each lane was loaded with 35 ?g of lysate. Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein. After blocking with 5% NGS and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot