Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- NB100-61666 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61666, RRID:AB_2150421
- Product name
- Goat Polyclonal NCF4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
KDFPEEDDPTN
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis.
Rioux JD, Xavier RJ, Taylor KD, Silverberg MS, Goyette P, Huett A, Green T, Kuballa P, Barmada MM, Datta LW, Shugart YY, Griffiths AM, Targan SR, Ippoliti AF, Bernard EJ, Mei L, Nicolae DL, Regueiro M, Schumm LP, Steinhart AH, Rotter JI, Duerr RH, Cho JH, Daly MJ, Brant SR
Nature genetics 2007 May;39(5):596-604
Nature genetics 2007 May;39(5):596-604
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCF4 Antibody [NB100-61666] - Analysis of NCF4 antibody using NB100-61666 at 0.01ug/ml in Daudi cell lysate (35ug protein in RIPA buffer).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCF4 Antibody [NB100-61666] - HEK293 overexpressing P40PHOX and probed with (mock transfection in first lane).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666] - (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666] - Staining of paraffin embedded Human Spleen at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.