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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB7315 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7315, RRID:AB_1577605
- Product name
- NCF4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of NCF4.
- Antigen sequence
C-KDFPEEDDPTN- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis.
Rioux JD, Xavier RJ, Taylor KD, Silverberg MS, Goyette P, Huett A, Green T, Kuballa P, Barmada MM, Datta LW, Shugart YY, Griffiths AM, Targan SR, Ippoliti AF, Bernard EJ, Mei L, Nicolae DL, Regueiro M, Schumm LP, Steinhart AH, Rotter JI, Duerr RH, Cho JH, Daly MJ, Brant SR
Nature genetics 2007 May;39(5):596-604
Nature genetics 2007 May;39(5):596-604
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NCF4 polyclonal antibody (Cat # PAB7315) (0.5 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- 293 overexpressing NCF4 and probed with NCF4 polyclonal antibody (Cat # PAB7315) (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NCF4 polyclonal antibody (Cat # PAB7315) (2.5 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
