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Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-42636 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCF4 Recombinant Rabbit Monoclonal Antibody (4L9Z4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: THP-1, Mouse thymus. The target is usually found in the following locations: Cytoplasm, Cytoplasmic side, Endosome membrane, Membrane, Peripheral membrane protein, cytosol. Immunogen sequence: LRCYYYEDTI STIKDIAVEE DLSSTPLLKD LLELTRREFQ REDIALNYRD AEGDLVRLLS DEDVALMVRQ ARGLPSQKRL FPWKLHITQK DNYRVYNTMP
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4L9Z4
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NCF4 in 300 μg extracts of THP-1 cells. Samples were precipitated with 3 μg NCF4 Monoclonal antibody (Product # MA5-42636). Western blot was performed from the immunoprecipitate using NCF4 Monoclonal antibody (Product # MA5-42636) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NCF4 in paraffin-embedded human colon carcinoma. Samples were incubated with NCF4 Monoclonal antibody (Product # MA5-42636) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
