PAB27877

antibody from Abnova Corporation

Targeting: BCL2L14 BCL-G, BCLG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27877

Provider

Abnova Corporation

Product name

BCL2L14 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant BCL2L14.

Antigen sequence

GQRTLEYQDSHSQQWSRCLSNVEQCLEHEAVDPKV
ISIANRVAEIVYSWPPPQATQAGGFKSKEIFVTEG
LSFQLQGHVPVASSSKKDEEEQILAKIVELL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408531) with BCL2L14 polyclonal antibody (Cat # PAB27877) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with BCL2L14 polyclonal antibody (Cat # PAB27877) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with BCL2L14 polyclonal antibody (Cat # PAB27877) shows moderate cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)