H00079370-M02

antibody from Abnova Corporation

Targeting: BCL2L14 BCL-G, BCLG

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00079370-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00079370-M02, RRID:AB_1112611

Product name

BCL2L14 monoclonal antibody (M02), clone 1D11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant BCL2L14.

Antigen sequence

MCSTSGCDLEEIPLDDDDLNTIEFKILAYYTRHHV
FKSTPALFSPKLLRTRSLSQRGLGNCSANESWTEV
SWPCRNSQSSEKAINLGKKKSSWKAFFGVVEKEDS
QSTPAKVSAQGQRTLEYQDSHSQQWSRCLSNVEQC
LEHEAVDPKVISIANRVAEIVYSWPPPQATQAGGF
KSKEIFVTEGLSFQLQGHVPVASSSKKDEEEQILA
KIVELLKYSGDQLERKLKKDKALMGHFQDGLSYSV
FKTITDQVLMGVDPRGESEVKAQGFKAALVIDVTA
KLTAIDNHPMNRVLGFGTKYLKENFSPWIQQHGGW
EKILGISHEEVD


Isotype

IgG

Antibody clone number

1D11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BCL2L14 expression in transfected 293T cell line by BCL2L14 monoclonal antibody (M02), clone 1D11.Lane 1: BCL2L14 transfected lysate(36.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BCL2L14 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of BCL2L14 transfected lysate using anti-BCL2L14 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BCL2L14 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol