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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-29025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHOX2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLaS3. Predicted reactivity: Mouse (100%), Xenopus laevis (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PHOX2B Polyclonal Antibody (Product # PA5-29025). Sample (30 µg whole cell lysate). A: HeLa S3. 12% SDS PAGE. PHOX2B Polyclonal Antibody (Product # PA5-29025) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PHOX2B Polyclonal Antibody detects PHOX2B protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHOX2B protein stained by PHOX2B Polyclonal Antibody (Product # PA5-29025) diluted at 1:1,000. Blue: Hoechst 33342 staining.
