AF-224-NA

antibody from Novus Biologicals

Targeting: IL2RB CD122, IL15RB

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-224-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-2 R beta Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human IL-2 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IL-2 R alpha , recombinant mouse (rm) IL-2 R beta , rhIL-2 R gamma , and rhIL-15 R is observed. Is also able to block the cell surface of human IL-2 R beta mediated bioactivities induced by IL-2. For optimal neutralization of IL-2 biological activity on cells expressing the high affinity IL-2 receptors, the use of anti-IL-2 R alpha in conjunction with anti-IL-2 R beta antibodies is recommended.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

250 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.

Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7221-9

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Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.

Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7221-9

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Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.

Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
Leukemia research 2004 Oct;28(10):1023-31

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 R beta Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-224-NA). The ND₅₀ is typically 10-30 µg/mL.