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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB224-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-2 R beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha , rh gamma c, rhIL-4 R, or rhIL-6 R is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-2 R beta in Human Lymph Node. IL-2 R beta was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human IL-2 R beta Monoclonal Antibody (Catalog # MAB224) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 R beta Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 R beta Monoclonal Antibody (Catalog # MAB224). The ND50 is typically 0.05-0.15 µg/mL.
