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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB224-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-2 R beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha , rh gamma c, rhIL-4 R, or rhIL-6 R is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_000869- Isotype
- IgG
- Antibody clone number
- 27302
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties.
Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection.
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X
Scientific reports 2016 Sep 2;6:32320
Scientific reports 2016 Sep 2;6:32320
A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties.
Ndhlovu LC, Leal FE, Eccles-James IG, Jha AR, Lanteri M, Norris PJ, Barbour JD, Wachter DJ, Andersson J, Taskén K, Torheim EA, Aandahl EM, Kallas EG, Nixon DF
European journal of immunology 2010 Jan;40(1):134-41
European journal of immunology 2010 Jan;40(1):134-41
Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7389-97
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7389-97
Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection.
Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M Jr, Lifson JD, Katsikis PD, Roederer M, Villinger F, Mattapallil JJ
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1439-48
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1439-48
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Miranda-Carús ME, Balsa A, Benito-Miguel M, Pérez de Ayala C, Martín-Mola E
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
Journal of immunology (Baltimore, Md. : 1950) 2004 Jul 15;173(2):1463-76
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-2 R beta in Human Lymph Node. IL-2 R beta was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human IL-2 R beta Monoclonal Antibody (Catalog # MAB224) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 R beta Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 R beta Monoclonal Antibody (Catalog # MAB224). The ND50 is typically 0.05-0.15 µg/mL.
