PA5-17358

antibody from Invitrogen Antibodies

Targeting: MALT1 MLT, PCASP1

Immunoprecipitation

Antibody data

Submit

Validation data

MALT1 protein structure - PA5-17358 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MALT1 in extracts from RL and A20 cell lines using MALT1 polyclonal antibody (Product # PA5-17358).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of MALT1 was achieved by transfecting HeLa with MALT1 specific siRNAs (Silencer® select Product # s21398). Western blot analysis (Fig. a) was performed using whole cell extracts from the MALT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with MALT1 Polyclonal Antibody (Product # PA5-17358, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MALT1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MALT1 Polyclonal Antibody (Product # PA5-17358) and a 92 kDa band corresponding to MALT1 along with an uncharacterized band (*) was observed in Hela, Raji, Jurkat and PC-3. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Raji (Lane 2), Jurkat (Lane 3) and PC-3 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).