Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- HPA023881 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA023881, RRID:AB_1857931
- Product name
- Anti-TFE3
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
SKDLESRQRSLEQANRSLQLRIQELELQAQIHGLP
VPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAA
TFHVGGGPAQNAPHQQPPAPPSDALLDLHFPSDHL
GDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLR
AAS- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references Resetting transcription factor control circuitry toward ground-state pluripotency in human.
Derivation of novel human ground state naive pluripotent stem cells
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3.
Inactivation of the FLCN tumor suppressor gene induces TFE3 transcriptional activity by increasing its nuclear localization.
Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
Cell 2014 Sep 11;158(6):1254-1269
Cell 2014 Sep 11;158(6):1254-1269
Derivation of novel human ground state naive pluripotent stem cells
Gafni O, Weinberger L, Mansour A, Manor Y, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna J
Nature 2013 October;504(7479):282-286
Nature 2013 October;504(7479):282-286
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3.
Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A
Cell 2013 Apr 11;153(2):335-47
Cell 2013 Apr 11;153(2):335-47
Inactivation of the FLCN tumor suppressor gene induces TFE3 transcriptional activity by increasing its nuclear localization.
Hong SB, Oh H, Valera VA, Baba M, Schmidt LS, Linehan WM
PloS one 2010 Dec 29;5(12):e15793
PloS one 2010 Dec 29;5(12):e15793
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TFE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
- Sample type
- HUMAN
Enhanced validation
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and pancreas tissues using HPA023881 antibody. Corresponding TFE3 RNA-seq data are presented for the same tissues.
- Sample type
- HUMAN
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human prostate shows moderate to strong nuclear positivity in glandular cells.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human kidney shows moderate nuclear positivity in renal glomeruli and tubuli cells.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human pancreas shows very weak nuclear positivity in exocrine glandular cells.
- Sample type
- HUMAN