Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [10]
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- Product number
- NBP1-82559 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82559, RRID:AB_11031626
- Product name
- Rabbit Polyclonal NKCC2/SLC12A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human NKCC2/SLC12A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice.
Harari-Steinberg O, Omer D, Gnatek Y, Pleniceanu O, Goldberg S, Cohen-Zontag O, Pri-Chen S, Kanter I, Ben Haim N, Becker E, Ankawa R, Fuchs Y, Kalisky T, Dotan Z, Dekel B
Cell reports 2020 Jan 21;30(3):852-869.e4
Cell reports 2020 Jan 21;30(3):852-869.e4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human kidney shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human lymph node shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum, kidney, liver and pancreas using Anti-SLC12A1 antibody NBP1-82559 (A) shows similar protein distribution across tissues to independent antibody NBP1-80993 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human kidney shows strong membranous positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining in human kidney and pancreas tissues using NBP1-82559 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.