M621B

antibody from Invitrogen Antibodies

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot

ELISA

Antibody data

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Validation data

Product number: M621B - Provider product page
Provider: Invitrogen Antibodies
Product name: IL-6 Monoclonal Antibody (7IL6), Biotin
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: M621B targets IL-6 in ELISA and WB applications and shows reactivity with Human samples. The M621B immunogen is recombinant human IL-6. M621B detects IL-6 which has a predicted molecular weight of approximately 22 kDa. The M621B IL6 antibody (biotinylated conjugate of clone 7IL6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M620 (clone 5IL6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody M621B (clone 7IL6) and antibody M620 (clone 5IL6) have successfully been used in combination with recombinant IL6 protein SIL6 in ELISA applications.
Reactivity: Human
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: 7IL6
Vial size: 250 µg
Concentration: 0.5 mg/mL
Storage: -20°C

IL6 protein structure - M621B shown in red.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Sandwich ELISA analysis of human IL-6 was performed using a Human IL-6 Colorimetric ELISA kit (Product # EH2IL6) by loading 50 µL per well of a mouse anti-human IL-6 biotinylated antibody (Product # M621B) at a concentration of 0.035 µg/mL and 50 µL of Human IL-6 Recombinant Protein (Product # SIL6) at 400, 160, 64, 25.6, 10.24, and 0 pg/mL (both in quadruplicate) across a 4 µg/mL mouse anti-human IL-6 (Product # M620) pre-coated plate. The plate was incubated for 2 hours at room temperature, washed, and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:7000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. NF-kappaB1, IL-6, MMP-13 and caspase-3 expression in the in vivo model of OA. (A) The relative protein expression levels of NF-kappaB1, IL-6, MMP-13 and caspase-3 were determined by western blot analysis in tissue samples from mice in the Sham, OA and OA + Res groups. Quantification of (B) NF-kappaB1, (C) IL-6, (D) MMP-13 and (E) caspase-3 protein expression. *P
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7. Effect of Res treatment on miR-9, NF-kappaB1, IL-6, MMP-13, caspase-3 expression in mouse chondrocytes. The relative (A) miR-9 and (B) NF-kappaB1 mRNA expression levels were determined by RT-qPCR in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. (C) The relative NF-kappaB1, IL-6, MMP-13 and caspase-3 protein expression levels were determined by western blot analysis in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. Quantification of (D) NF-kappaB1, (E) IL-6, (F) MMP-13 and (G) caspase-3 protein expression. n=3. *P
Conjugate: Biotin