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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- IC206P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity.
TLR costimulation causes oxidative stress with unbalance of proinflammatory and anti-inflammatory cytokine production.
A novel population of human CD56+ human leucocyte antigen D-related (HLA-DR+) colonic lamina propria cells is associated with inflammation in ulcerative colitis.
Piancone F, Saresella M, Marventano I, La Rosa F, Zoppis M, Agostini S, Longhi R, Caputo D, Mendozzi L, Rovaris M, Clerici M
Scientific reports 2016 Jul 14;6:29699
Scientific reports 2016 Jul 14;6:29699
TLR costimulation causes oxidative stress with unbalance of proinflammatory and anti-inflammatory cytokine production.
Lavieri R, Piccioli P, Carta S, Delfino L, Castellani P, Rubartelli A
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5373-81
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5373-81
A novel population of human CD56+ human leucocyte antigen D-related (HLA-DR+) colonic lamina propria cells is associated with inflammation in ulcerative colitis.
Ng SC, Plamondon S, Al-Hassi HO, English N, Gellatly N, Kamm MA, Knight SC, Stagg AJ
Clinical and experimental immunology 2009 Nov;158(2):205-18
Clinical and experimental immunology 2009 Nov;158(2):205-18
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IL-6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL-6 PE-conjugated Monoclonal Antibody (Catalog # IC206P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
