GTX15816

antibody from GeneTex

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

GTX15816

Provider

GeneTex

Product name

IL6 antibody [20F3] (Biotin)

Antibody type

Monoclonal

Reactivity

Mouse

Host

Rat

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sandwich ELISA analysis of mouse IL6 in dodecuplicate at 2000, 800, 320, 128, 51, and 0 pg/ml (50 ul per well) using IL6 antibody [32C11] as capture antibody (3 ug/ml) and IL6 antibody [20F3] (Biotin) as detection antibody (0.25 ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.