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- References [16]
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- Product number
- AHC0562 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-6 Monoclonal Antibody (677B6A2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is recommended as a capture antibody in Sandwich ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 677B6A2
- Vial size
- 500 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits.
Atomic force microscopy analysis of IgG films at hydrophobic surfaces: a promising method to probe IgG orientations and optimize ELISA tests performance.
Dopamine receptors in human adipocytes: expression and functions.
Rheumatoid factor and its interference with cytokine measurements: problems and solutions.
Neuroinflammation in delirium: a postmortem case-control study.
Insulin stimulates interleukin-6 expression and release in LS14 human adipocytes through multiple signaling pathways.
Interleukin-17 in transverse myelitis and multiple sclerosis.
Astrocytes produce interferon-alpha and CXCL10, but not IL-6 or CXCL8, in Aicardi-Goutières syndrome.
Assessment of endoscopic activity index and biological inflammatory markers in clinically active Crohn's disease with normal C-reactive protein serum level.
Interleukin-12 is involved in the enhancement of human natural killer cell activity by Lactobacillus casei Shirota.
Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells.
Biological characterization of lipopolysaccharide from Treponema pectinovorum.
Biological characterization of lipopolysaccharide from Treponema pectinovorum.
Igf-I inhibits spontaneous apoptosis in human granulocytes.
Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.
Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.
Rodriguez E, Boelaars K, Brown K, Madunić K, van Ee T, Dijk F, Verheij J, Li RJE, Schetters STT, Meijer LL, Le Large TYS, Driehuis E, Clevers H, Bruijns SCM, O'Toole T, van Vliet SJ, Bijlsma MF, Wuhrer M, Kazemier G, Giovannetti E, Garcia-Vallejo JJ, van Kooyk Y
Communications biology 2022 Jan 11;5(1):41
Communications biology 2022 Jan 11;5(1):41
Atomic force microscopy analysis of IgG films at hydrophobic surfaces: a promising method to probe IgG orientations and optimize ELISA tests performance.
de Thier P, Bacharouche J, Duval JF, Skali-Lami S, Francius G
Biochimica et biophysica acta 2015 Feb;1854(2):138-45
Biochimica et biophysica acta 2015 Feb;1854(2):138-45
Dopamine receptors in human adipocytes: expression and functions.
Borcherding DC, Hugo ER, Idelman G, De Silva A, Richtand NW, Loftus J, Ben-Jonathan N
PloS one 2011;6(9):e25537
PloS one 2011;6(9):e25537
Rheumatoid factor and its interference with cytokine measurements: problems and solutions.
Bartels EM, Falbe Wätjen I, Littrup Andersen E, Danneskiold-Samsøe B, Bliddal H, Ribel-Madsen S
Arthritis 2011;2011:741071
Arthritis 2011;2011:741071
Neuroinflammation in delirium: a postmortem case-control study.
Munster BC, Aronica E, Zwinderman AH, Eikelenboom P, Cunningham C, Rooij SE
Rejuvenation research 2011 Dec;14(6):615-22
Rejuvenation research 2011 Dec;14(6):615-22
Insulin stimulates interleukin-6 expression and release in LS14 human adipocytes through multiple signaling pathways.
LaPensee CR, Hugo ER, Ben-Jonathan N
Endocrinology 2008 Nov;149(11):5415-22
Endocrinology 2008 Nov;149(11):5415-22
Interleukin-17 in transverse myelitis and multiple sclerosis.
Graber JJ, Allie SR, Mullen KM, Jones MV, Wang T, Krishnan C, Kaplin AI, Nath A, Kerr DA, Calabresi PA
Journal of neuroimmunology 2008 May 30;196(1-2):124-32
Journal of neuroimmunology 2008 May 30;196(1-2):124-32
Astrocytes produce interferon-alpha and CXCL10, but not IL-6 or CXCL8, in Aicardi-Goutières syndrome.
van Heteren JT, Rozenberg F, Aronica E, Troost D, Lebon P, Kuijpers TW
Glia 2008 Apr;56(5):568-78
Glia 2008 Apr;56(5):568-78
Assessment of endoscopic activity index and biological inflammatory markers in clinically active Crohn's disease with normal C-reactive protein serum level.
Denis MA, Reenaers C, Fontaine F, Belaïche J, Louis E
Inflammatory bowel diseases 2007 Sep;13(9):1100-5
Inflammatory bowel diseases 2007 Sep;13(9):1100-5
Interleukin-12 is involved in the enhancement of human natural killer cell activity by Lactobacillus casei Shirota.
Takeda K, Suzuki T, Shimada SI, Shida K, Nanno M, Okumura K
Clinical and experimental immunology 2006 Oct;146(1):109-15
Clinical and experimental immunology 2006 Oct;146(1):109-15
Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells.
Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA
Immunology 2006 Jul;118(3):353-60
Immunology 2006 Jul;118(3):353-60
Biological characterization of lipopolysaccharide from Treponema pectinovorum.
Kesavalu L, Falk CW, Davis KJ, Steffen MJ, Xu X, Holt SC, Ebersole JL
Infection and immunity 2002 Jan;70(1):211-7
Infection and immunity 2002 Jan;70(1):211-7
Biological characterization of lipopolysaccharide from Treponema pectinovorum.
Kesavalu L, Falk CW, Davis KJ, Steffen MJ, Xu X, Holt SC, Ebersole JL
Infection and immunity 2002 Jan;70(1):211-7
Infection and immunity 2002 Jan;70(1):211-7
Igf-I inhibits spontaneous apoptosis in human granulocytes.
Kooijman R, Coppens A, Hooghe-Peters E
Endocrinology 2002 Apr;143(4):1206-12
Endocrinology 2002 Apr;143(4):1206-12
Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.
Nixon CS, Steffen MJ, Ebersole JL
Infection and immunity 2000 Sep;68(9):5284-92
Infection and immunity 2000 Sep;68(9):5284-92
Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.
Nixon CS, Steffen MJ, Ebersole JL
Infection and immunity 2000 Sep;68(9):5284-92
Infection and immunity 2000 Sep;68(9):5284-92
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Representative Standard Curve Invitrogen vs. competitor human IL-6 ELISA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effects of DA on adipokine/cytokine release. Sc explants ( panel A ), isolated mature adipocytes ( Panel B ) or differentiated primary adipocytes ( panel C ) were incubated with 1 or 100 nM DA or with 10 nM of SKF38393, a D1R/D5R agonist, for 24 hr. Leptin concentration in CM was determined by ELISA. Each value is a mean+-SEM of 5 determinations; *, p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Time- and dose-dependent effect of insulin on IL-6 release and gene expression in undifferentiated LS14 human adipocytes. A, Cells were treated with insulin for 6 h. B, Cells were incubated with 125 nm insulin (black circles) or 10 ng/ml TNFα (white triangles) for different times. IL-6 in CM was measured by ELISA. C, Cells were incubated with 125 nm insulin, and IL-6 mRNA levels were determined by real-time PCR. Values in A and B are the mean ± sem of four determinations, and are representative of three separate experiments. IL-6 gene expression was calculated as the fold change over controls and represents three separate experiments. *, Significant differences (P < 0.05) from control.
