Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- AHC0762 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-6 Monoclonal Antibody (8H12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8H12
- Vial size
- 250 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction.
Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.
Back J, Nguyen MN, Li L, Lee S, Lee I, Chen F, Gillinov L, Chung YH, Alder KD, Kwon HK, Yu KE, Dussik CM, Hao Z, Flores MJ, Kim Y, Ibe IK, Munger AM, Seo SW, Lee FY
Bone research 2021 Sep 29;9(1):43
Bone research 2021 Sep 29;9(1):43
Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.
Buken C, Sahana J, Corydon TJ, Melnik D, Bauer J, Wehland M, Krüger M, Balk S, Abuagela N, Infanger M, Grimm D
Scientific reports 2019 Aug 15;9(1):11882
Scientific reports 2019 Aug 15;9(1):11882
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry analysis of IL-6 showing staining in the cytoplasm of paraffin-embedded human spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a IL-6 monoclonal antibody (Product # AHC0762) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.