Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB1609 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects canine IL-6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Cardiotrophin-1, recombinant human (rh) CLC, recombinant rat (rr) CNTF, recombinant cotton rat IL-6, recombinant equine IL-6, rhIL-6, recombinant porcine IL-6, rrIL-6, rmIL-11, rmLIF, or rmOncostatin M is observed.
- Reactivity
- Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Canine IL-6 Antibody. Recombinant Canine IL-6 (Catalog # 1609-CL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB1609). The ND50 is typically 10-30 µg/mL.