Antibody data
- Antibody Data
- Antigen structure
- References [33]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB406-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-6, recombinant porcine IL-6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- MP5-20F3
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.
Phosphorylation of Hsp20 Promotes Fibrotic Remodeling and Heart Failure.
Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulating adipose stromal interleukin 6 production in mice.
Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells.
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6.
IL6 Blockade Reprograms the Lung Tumor Microenvironment to Limit the Development and Progression of K-ras-Mutant Lung Cancer.
Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis.
The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity.
Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling.
Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
Oxidative stress-induced inhibition of Sirt1 by caveolin-1 promotes p53-dependent premature senescence and stimulates the secretion of interleukin 6 (IL-6).
Stabilization and augmentation of circulating AIM in mice by synthesized IgM-Fc.
Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels.
Catecholamine stress alters neutrophil trafficking and impairs wound healing by β2-adrenergic receptor-mediated upregulation of IL-6.
Basophils control T-cell responses and limit disease activity in experimental murine colitis.
Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Maternal immune activation alters fetal brain development through interleukin-6.
Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
Sequential ELISA to profile multiple cytokines from small volumes.
Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation.
Selective macrophage suppression during sepsis.
Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways.
Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes.
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N
Oncogene 2019 May;38(22):4366-4383
Oncogene 2019 May;38(22):4366-4383
Phosphorylation of Hsp20 Promotes Fibrotic Remodeling and Heart Failure.
Gardner GT, Travers JG, Qian J, Liu GS, Haghighi K, Robbins N, Jiang M, Li Y, Fan GC, Rubinstein J, Blaxall BC, Kranias EG
JACC. Basic to translational science 2019 Apr;4(2):188-199
JACC. Basic to translational science 2019 Apr;4(2):188-199
Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulating adipose stromal interleukin 6 production in mice.
Sun X, Gupta K, Wu B, Zhang D, Yuan B, Zhang X, Chiang HC, Zhang C, Curiel TJ, Bendeck MP, Hursting S, Hu Y, Li R
The Journal of biological chemistry 2018 Feb 23;293(8):2841-2849
The Journal of biological chemistry 2018 Feb 23;293(8):2841-2849
Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells.
Akama T, Chun TH
The Journal of biological chemistry 2018 Apr 27;293(17):6603-6610
The Journal of biological chemistry 2018 Apr 27;293(17):6603-6610
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Augmented sphingosine 1 phosphate receptor-1 signaling in cardiac fibroblasts induces cardiac hypertrophy and fibrosis through angiotensin II and interleukin-6.
Ohkura SI, Usui S, Takashima SI, Takuwa N, Yoshioka K, Okamoto Y, Inagaki Y, Sugimoto N, Kitano T, Takamura M, Wada T, Kaneko S, Takuwa Y
PloS one 2017;12(8):e0182329
PloS one 2017;12(8):e0182329
IL6 Blockade Reprograms the Lung Tumor Microenvironment to Limit the Development and Progression of K-ras-Mutant Lung Cancer.
Caetano MS, Zhang H, Cumpian AM, Gong L, Unver N, Ostrin EJ, Daliri S, Chang SH, Ochoa CE, Hanash S, Behrens C, Wistuba II, Sternberg C, Kadara H, Ferreira CG, Watowich SS, Moghaddam SJ
Cancer research 2016 Jun 1;76(11):3189-99
Cancer research 2016 Jun 1;76(11):3189-99
Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis.
Lim JY, Im KI, Lee ES, Kim N, Nam YS, Jeon YW, Cho SG
Scientific reports 2016 Jun 1;6:26851
Scientific reports 2016 Jun 1;6:26851
The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity.
Lazzarini E, Balbi C, Altieri P, Pfeffer U, Gambini E, Canepa M, Varesio L, Bosco MC, Coviello D, Pompilio G, Brunelli C, Cancedda R, Ameri P, Bollini S
Scientific reports 2016 Jul 21;6:29994
Scientific reports 2016 Jul 21;6:29994
Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling.
Chandler RL, Damrauer JS, Raab JR, Schisler JC, Wilkerson MD, Didion JP, Starmer J, Serber D, Yee D, Xiong J, Darr DB, Pardo-Manuel de Villena F, Kim WY, Magnuson T
Nature communications 2015 Jan 27;6:6118
Nature communications 2015 Jan 27;6:6118
Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F
EMBO molecular medicine 2015 Feb;7(2):211-26
EMBO molecular medicine 2015 Feb;7(2):211-26
Oxidative stress-induced inhibition of Sirt1 by caveolin-1 promotes p53-dependent premature senescence and stimulates the secretion of interleukin 6 (IL-6).
Volonte D, Zou H, Bartholomew JN, Liu Z, Morel PA, Galbiati F
The Journal of biological chemistry 2015 Feb 13;290(7):4202-14
The Journal of biological chemistry 2015 Feb 13;290(7):4202-14
Stabilization and augmentation of circulating AIM in mice by synthesized IgM-Fc.
Kai T, Yamazaki T, Arai S, Miyazaki T
PloS one 2014;9(5):e97037
PloS one 2014;9(5):e97037
Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels.
Ke F, Zhang L, Liu Z, Liu J, Yan S, Xu Z, Bai J, Zhu H, Lou F, Wang H, Shi Y, Jiang Y, Su B, Wang H
Stem cells (Dayton, Ohio) 2014 Oct;32(10):2799-810
Stem cells (Dayton, Ohio) 2014 Oct;32(10):2799-810
Catecholamine stress alters neutrophil trafficking and impairs wound healing by β2-adrenergic receptor-mediated upregulation of IL-6.
Kim MH, Gorouhi F, Ramirez S, Granick JL, Byrne BA, Soulika AM, Simon SI, Rivkah Isseroff R
The Journal of investigative dermatology 2014 Mar;134(3):809-817
The Journal of investigative dermatology 2014 Mar;134(3):809-817
Basophils control T-cell responses and limit disease activity in experimental murine colitis.
Gomez MR, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, Goebel N, Schmidbauer K, Dunger N, Brühl H, Renner K, Syed SN, Mack M
Mucosal immunology 2014 Jan;7(1):188-99
Mucosal immunology 2014 Jan;7(1):188-99
Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
Ramkhelawon B, Hennessy EJ, Ménager M, Ray TD, Sheedy FJ, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner KJ, Moore KJ
Nature medicine 2014 Apr;20(4):377-84
Nature medicine 2014 Apr;20(4):377-84
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Junkins RD, MacNeil AJ, Wu Z, McCormick C, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
Infection and immunity 2012 Jan;80(1):410-7
Infection and immunity 2012 Jan;80(1):410-7
Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesnière A, Ibrahim N, Déchanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
The Journal of experimental medicine 2011 Mar 14;208(3):491-503
The Journal of experimental medicine 2011 Mar 14;208(3):491-503
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
Microbes and infection 2009 Apr;11(4):524-30
Microbes and infection 2009 Apr;11(4):524-30
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology 2008 Oct;125(2):272-9
Immunology 2008 Oct;125(2):272-9
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
Maternal immune activation alters fetal brain development through interleukin-6.
Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 3;27(40):10695-702
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 3;27(40):10695-702
Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
Bone 2007 May;40(5):1276-83
Bone 2007 May;40(5):1276-83
A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
Journal of molecular and cellular cardiology 2006 Nov;41(5):885-92
Journal of molecular and cellular cardiology 2006 Nov;41(5):885-92
Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
Journal of ethnopharmacology 2006 Jul 19;106(3):418-24
Journal of ethnopharmacology 2006 Jul 19;106(3):418-24
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation.
Fredj S, Bescond J, Louault C, Delwail A, Lecron JC, Potreau D
Journal of cellular physiology 2005 Aug;204(2):428-36
Journal of cellular physiology 2005 Aug;204(2):428-36
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways.
Miyamoto M, Prause O, Sjöstrand M, Laan M, Lötvall J, Lindén A
Journal of immunology (Baltimore, Md. : 1950) 2003 May 1;170(9):4665-72
Journal of immunology (Baltimore, Md. : 1950) 2003 May 1;170(9):4665-72
Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes.
Pechová J, Stĕpánová G, Kovár L, Kopecký J
Folia parasitologica 2002;49(2):153-9
Folia parasitologica 2002;49(2):153-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Mouse IL-6 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-6 (Catalog # 406-ML), Recombinant Human IL-6 (Catalog # 206-IL) and Recombinant Rat IL-6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-6 Monoclonal Antibody (Catalog # MAB406) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Mouse IL-6 Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6 Monoclonal Antibody (Catalog # MAB406). The ND50 is typically 0.005-0.025 µg/mL.