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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAF1609 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine IL-6 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine IL-6 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant feline IL-6 and recombinant porcine IL-6 is observed and less than 0.2% cross-reactivity with recombinant human IL-6, recombinant mouse IL-6, recombinant rat IL-6, recombinant cotton rat IL-6, and recombinant equine IL-6 is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P41323- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
Wood MW, Breitschwerdt EB, Gookin JL
Infection and immunity 2011 Feb;79(2):708-15
Infection and immunity 2011 Feb;79(2):708-15
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-6 in Canine PBMCs. IL-6 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with Concanavalin A using Goat Anti-Canine IL-6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1609) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
