IC2062G-025

antibody from R&D Systems

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC2062G-025

Provider

R&D Systems

Product name

Human IL-6 Alexa Fluor® 488-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human IL-6 in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antigen sequence

Q75MH2


Isotype

IgG

Antibody clone number

903129

Vial size

25 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) untreated or (B) treated overnight with 250 ng/mL LPS were stained with Mouse Anti-Human IL-6 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC2062G) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.