Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF506 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IL-6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat and mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant cotton rat IL-6 is observed, and less than 1% cross-reactivity with recombinant human IL-6 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P20607
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Upregulation of CD81 in trophoblasts induces an imbalance of Treg/Th17 cells by promoting IL-6 expression in preeclampsia.
Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection.
Mesenchymal stem cells and cell-derived extracellular vesicles protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers.
Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model.
Early CALP2 expression and microglial activation are potential inducers of spinal IL-6 up-regulation and bilateral pain following motor nerve injury.
Repeated water avoidance stress induces visceral hypersensitivity: Role of interleukin-1, interleukin-6, and peripheral corticotropin-releasing factor.
Mu-opioid receptor and delta-opioid receptor differentially regulate microglial inflammatory response to control proopiomelanocortin neuronal apoptosis in the hypothalamus: effects of neonatal alcohol.
Mechanisms involved in dual vasopressin/apelin neuron dysfunction during aging.
Sublytic C5b-9 induces IL-6 and TGF-β1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPβ acetylation.
Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop.
Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
Fetal asphyctic preconditioning modulates the acute cytokine response thereby protecting against perinatal asphyxia in neonatal rats.
Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus.
Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness).
JAK/STAT3 pathway is activated in spinal cord microglia after peripheral nerve injury and contributes to neuropathic pain development in rat.
Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha.
Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells.
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
Mechanism of IL-6-mediated cardiac dysfunction following trauma-hemorrhage.
Ding H, Dai Y, Lei Y, Wang Z, Liu D, Li R, Shen L, Gu N, Zheng M, Zhu X, Zhao G, Hu Y
Cellular & molecular immunology 2019 Jan;16(1):302-312
Cellular & molecular immunology 2019 Jan;16(1):302-312
Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection.
Lai HC, Lu CH, Wong CS, Lin BF, Chan SM, Kuo CY, Wu ZF
Journal of the Chinese Medical Association : JCMA 2018 Nov;81(11):955-963
Journal of the Chinese Medical Association : JCMA 2018 Nov;81(11):955-963
Mesenchymal stem cells and cell-derived extracellular vesicles protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers.
de Godoy MA, Saraiva LM, de Carvalho LRP, Vasconcelos-Dos-Santos A, Beiral HJV, Ramos AB, Silva LRP, Leal RB, Monteiro VHS, Braga CV, de Araujo-Silva CA, Sinis LC, Bodart-Santos V, Kasai-Brunswick TH, Alcantara CL, Lima APCA, da Cunha-E Silva NL, Galina A, Vieyra A, De Felice FG, Mendez-Otero R, Ferreira ST
The Journal of biological chemistry 2018 Feb 9;293(6):1957-1975
The Journal of biological chemistry 2018 Feb 9;293(6):1957-1975
Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model.
Chong SA, Balosso S, Vandenplas C, Szczesny G, Hanon E, Claes K, Van Damme X, Danis B, Van Eyll J, Wolff C, Vezzani A, Kaminski RM, Niespodziany I
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2018 Apr;15(2):470-488
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2018 Apr;15(2):470-488
Early CALP2 expression and microglial activation are potential inducers of spinal IL-6 up-regulation and bilateral pain following motor nerve injury.
Chen SX, Wang SK, Yao PW, Liao GJ, Na XD, Li YY, Zeng WA, Liu XG, Zang Y
Journal of neurochemistry 2018 Apr;145(2):154-169
Journal of neurochemistry 2018 Apr;145(2):154-169
Repeated water avoidance stress induces visceral hypersensitivity: Role of interleukin-1, interleukin-6, and peripheral corticotropin-releasing factor.
Nozu T, Miyagishi S, Nozu R, Takakusaki K, Okumura T
Journal of gastroenterology and hepatology 2017 Dec;32(12):1958-1965
Journal of gastroenterology and hepatology 2017 Dec;32(12):1958-1965
Mu-opioid receptor and delta-opioid receptor differentially regulate microglial inflammatory response to control proopiomelanocortin neuronal apoptosis in the hypothalamus: effects of neonatal alcohol.
Shrivastava P, Cabrera MA, Chastain LG, Boyadjieva NI, Jabbar S, Franklin T, Sarkar DK
Journal of neuroinflammation 2017 Apr 14;14(1):83
Journal of neuroinflammation 2017 Apr 14;14(1):83
Mechanisms involved in dual vasopressin/apelin neuron dysfunction during aging.
Sauvant J, Delpech JC, Palin K, De Mota N, Dudit J, Aubert A, Orcel H, Roux P, Layé S, Moos F, Llorens-Cortes C, Nadjar A
PloS one 2014;9(2):e87421
PloS one 2014;9(2):e87421
Sublytic C5b-9 induces IL-6 and TGF-β1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPβ acetylation.
Zhang J, Li Y, Shan K, Wang L, Qiu W, Lu Y, Zhao D, Zhu G, He F, Wang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1511-25
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1511-25
Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Shigemoto-Mogami Y, Hoshikawa K, Goldman JE, Sekino Y, Sato K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2231-43
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2231-43
Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop.
Bustamante M, Fernández-Verdejo R, Jaimovich E, Buvinic S
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E869-82
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E869-82
Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
Furuhashi K, Tsuboi N, Shimizu A, Katsuno T, Kim H, Saka Y, Ozaki T, Sado Y, Imai E, Matsuo S, Maruyama S
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):587-603
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):587-603
Fetal asphyctic preconditioning modulates the acute cytokine response thereby protecting against perinatal asphyxia in neonatal rats.
Vlassaks E, Strackx E, Vles JSh, Nikiforou M, Martinez-Martinez P, Kramer BW, Gavilanes AW
Journal of neuroinflammation 2013 Jan 26;10:14
Journal of neuroinflammation 2013 Jan 26;10:14
Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus.
Oh J, McCloskey MA, Blong CC, Bendickson L, Nilsen-Hamilton M, Sakaguchi DS
Journal of neuroscience research 2010 Oct;88(13):2798-809
Journal of neuroscience research 2010 Oct;88(13):2798-809
Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness).
Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Mar 10;30(10):3752-61
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Mar 10;30(10):3752-61
JAK/STAT3 pathway is activated in spinal cord microglia after peripheral nerve injury and contributes to neuropathic pain development in rat.
Dominguez E, Rivat C, Pommier B, Mauborgne A, Pohl M
Journal of neurochemistry 2008 Oct;107(1):50-60
Journal of neurochemistry 2008 Oct;107(1):50-60
Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha.
Xu J, Kim HT, Ma Y, Zhao L, Zhai L, Kokorina N, Wang P, Messina JL
Endocrinology 2008 May;149(5):2369-82
Endocrinology 2008 May;149(5):2369-82
Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells.
Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H
The European journal of neuroscience 2007 Feb;25(3):649-58
The European journal of neuroscience 2007 Feb;25(3):649-58
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
Barkho BZ, Song H, Aimone JB, Smrt RD, Kuwabara T, Nakashima K, Gage FH, Zhao X
Stem cells and development 2006 Jun;15(3):407-21
Stem cells and development 2006 Jun;15(3):407-21
Mechanism of IL-6-mediated cardiac dysfunction following trauma-hemorrhage.
Yang S, Hu S, Hsieh YC, Choudhry MA, Rue LW 3rd, Bland KI, Chaudry IH
Journal of molecular and cellular cardiology 2006 Apr;40(4):570-9
Journal of molecular and cellular cardiology 2006 Apr;40(4):570-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat IL-6 by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6 in Mouse T Cells. IL-6 was detected in immersion fixed activated mouse T Cells using 15 µg/mL Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Rat IL-6 Antibody. Recombinant Rat IL-6 (Catalog # 506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND50 is typically 0.1-0.5 µg/mL.