LS-C154128

antibody from LSBio

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C154128

Provider

LSBio

Product name

IL6 / Interleukin 6 Antibody LS-C154128

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Storage

Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Mouse IL-6 Antibody - Western Blot. Anti-mouse IL-6 antibody in western blot shows detection of recombinant mouse IL-6 raised in E. coli. Recombinant truncated protein (0.1 ug, 21.7 kD) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry with anti-IL-6 antibody showing nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry with anti-IL-6 antibody showing nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry with anti-IL-6 antibody showing nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.