ABIN2508605

antibody from antibodies-online

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Antibody data

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Validation data

Product number: ABIN2508605 - Provider product page
Provider: antibodies-online
Product name: anti-Interleukin 6 (IL6) antibody (Biotin)
Antibody type: Polyclonal
Antigen: Other
Description: Produced from sera of goats pre-immunized with highly pure recombinant Human IL-6. Anti-Human IL-6 specific antibody was purified by affinity chromatography and then biotinylated.
Reactivity: Human
Host: Goat
Conjugate: Biotin
Vial size: 50 μg
Storage: -20°C

IL6 protein structure - ABIN2508605 shown in red.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6): 1, 2, 3. Anti-Melanocortin Receptor 1 antibody (ABIN2511173), (1:500). 4, 5, 6. Anti-Melanocortin Receptor 1 antibody, preincubated with the control peptide antigen Western blot analysis of rat adrenal lysate: 1. Anti-Melanocortin Receptor 1 antibody (ABIN2511173), (1:400). 2. Anti-Melanocortin Receptor 1 antibody, preincubated with the control peptide antigen. Expression of MC1R in normal skin and melanoma Immunohistochemical staining of paraffin embedded normal skin and melanoma sections using Anti-Melanocortin Receptor 1 antibody (ABIN2511173) (1:100). MCR1 staining (red-brown color is highly specific in A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells. Color reaction was obtained with DAB. Hematoxilin is used as the counterstain. Expression of MC1R in human Malme-3M cells Immunocytochemical staining of human paraformaldehyde fixed and permeabilized malignant melanoma cell lines (Malme-3M). A. Cells were stained with Anti-Melanocortin Receptor 1 antibody (ABIN2511173), (1:200) followed by goat-anti-rabbit- AlexaFluor-555 secondary antibody. B. Live view of the same field as in (A). C. Computer merge of (A) and (B).

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6): 1, 2, 3. Anti-Melanocortin Receptor 1 antibody (ABIN2511173), (1:500). 4, 5, 6. Anti-Melanocortin Receptor 1 antibody, preincubated with the control peptide antigen Western blot analysis of rat adrenal lysate: 1. Anti-Melanocortin Receptor 1 antibody (ABIN2511173), (1:400). 2. Anti-Melanocortin Receptor 1 antibody, preincubated with the control peptide antigen. Expression of MC1R in normal skin and melanoma Immunohistochemical staining of paraffin embedded normal skin and melanoma sections using Anti-Melanocortin Receptor 1 antibody (ABIN2511173) (1:100). MCR1 staining (red-brown color is highly specific in A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells. Color reaction was obtained with DAB. Hematoxilin is used as the counterstain. Expression of MC1R in human Malme-3M cells Immunocytochemical staining of human paraformaldehyde fixed and permeabilized malignant melanoma cell lines (Malme-3M). A. Cells were stained with Anti-Melanocortin Receptor 1 antibody (ABIN2511173), (1:200) followed by goat-anti-rabbit- AlexaFluor-555 secondary antibody. B. Live view of the same field as in (A). C. Computer merge of (A) and (B).