AP52199PU-N

antibody from OriGene

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP52199PU-N

Provider

OriGene

Product name

IL6 (Center) rabbit polyclonal antibody, Aff - Purified

Antibody type

Polyclonal

Description

IL6 (Center) rabbit polyclonal antibody, Aff - Purified

Host

Rabbit

Conjugate

Unconjugated

Epitope

IL6

Antibody clone number

NULL

Vial size

400 µl

Concentration

lot specific

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of Interleukin-6 (IL6)?Antibody (Center) ?in NCI-H460 cell line lysates (35ug/lane). This demonstrates the IL6 antibody detected the IL6 protein (arrow).

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with Interleukin-6 (IL6)?Antibody (Center) ??followed by peroxidase conjugation of the secondary antibody and DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of NCI-H460 cells using Interleukin-6 (IL6)?Antibody (Center) ??(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

FC