Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB9540-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-6 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P05231
- Isotype
- IgG
- Antibody clone number
- 973132
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human IL-6 ELISA Standard Curve. Recombinant human IL-6 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB9540) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2063) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).