TA500065

antibody from OriGene

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500065

Provider

OriGene

Proper citation

OriGene Cat#TA500065, RRID:AB_2055829

Product name

IL6 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)

Antibody type

Monoclonal

Description

IL6 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)

Host

Mouse

Conjugate

Unconjugated

Epitope

IL6

Isotype

IgG

Antibody clone number

OTI2C9

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (RC202078, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IL-6 antibody (2C9) at 1:50000 + recombinant human IL-6 (Cat# RC202078) IL-6 antibody (2G3) at 1:2000

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500065)

Validation comment

IHC