109-401-310

antibody from Rockland Immunochemicals, Inc.

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

109-401-310

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#109-401-310, RRID:AB_218931

Product name

Anti-Human IL-6 (RABBIT) Antibody - 109-401-310

Antibody type

Polyclonal

Vial size

1 µl

References

Submitted references

Preliminary Report: The Relevance of Tumor Necrosis Factor-α in Acquired Primary Lymphedema-A Histopathological Investigation.

Asano S, Mikami T, Matsubara S, Maegawa J, Wakui H, Tamura K, Yoshimi R
Lymphatic research and biology 2020 Jun;18(3):232-238

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Inducement of mitogen-activated protein kinases in frozen shoulders.

Kanbe K, Inoue K, Inoue Y, Chen Q
Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2009 Jan;14(1):56-61

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

Validation comment

Western Blot