Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP52199PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52199PU-N, RRID:AB_11148518
- Product name
- anti Interleukin-6 (IL6) (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 26-55 amino acids from the Central region of Human Interleukin-6
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Interleukin-6 (IL6)Â Antibody (Center) Cat.-No AP52199PU-NÂ in NCI-H460 cell line lysates (35ug/lane). This demonstrates the IL6 antibody detected the IL6 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with Interleukin-6 (IL6) Antibody (Center) Cat.-No AP52199PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using Interleukin-6 (IL6) Antibody (Center) Cat.-No AP52199PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.