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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Flow cytometry [1]
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- Product number
- IC206F - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-6 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P05231- Isotype
- IgG
- Antibody clone number
- 1936
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients.
Expression of the T cell receptor αβ on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Kinin receptor expression during Staphylococcus aureus infection.
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Gironi M, Saresella M, Rovaris M, Vaghi M, Nemni R, Clerici M, Grossi E
Immunity & ageing : I & A 2013 Jan 10;10(1):1
Immunity & ageing : I & A 2013 Jan 10;10(1):1
Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients.
Al-Daghri NM, Al-Attas O, Alokail MS, Alkharfy KM, Draz HM, Agliardi C, Mohammed AK, Guerini FR, Clerici M
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1325-32
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1325-32
Expression of the T cell receptor αβ on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
PloS one 2011 Jan 11;6(1):e15997
PloS one 2011 Jan 11;6(1):e15997
Kinin receptor expression during Staphylococcus aureus infection.
Bengtson SH, Phagoo SB, Norrby-Teglund A, Påhlman L, Mörgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
Blood 2006 Sep 15;108(6):2055-63
Blood 2006 Sep 15;108(6):2055-63
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
FEBS letters 2003 Mar 13;538(1-3):149-54
FEBS letters 2003 Mar 13;538(1-3):149-54
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
FEBS letters 2003 Mar 13;538(1-3):149-54
FEBS letters 2003 Mar 13;538(1-3):149-54
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL-6 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC206F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
