200-301-F31

antibody from Rockland Immunochemicals, Inc.

Targeting: GABRB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

200-301-F31

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-F31, RRID:AB_2611153

Product name

Anti-GABA-A Receptor BETA 1 (MOUSE) Monoclonal Antibody - 200-301-F31

Antibody type

Monoclonal

Description

Anti-GABA-A receptor Beta1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with GABA-A receptor Beta1 from human, mouse, and rat based on 100% homology with the immunizing sequence. No cross-reactivity against GABA-A-R-Beta 2 or –Beta3. Cross-reactivity with GABA-A receptor Beta1 from other sources has not been determined. Ion Channels research.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

S96-55

Vial size

100 µl

Concentration

1.0 mg/ml

Storage

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Handling

Dry Ice

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of mouse anti-GABA-A Receptor BETA 1 antibody. Lane 1: human cell line mix. Lane 2: none. Load: 35 µg per lane. Primary antibody: GABA-A Receptor BETA 1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 54.1 kDa, ~55 kDa for GABA-A Receptor BETA 1. Other band(s): none.

Validation comment

Western Blot