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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA300039 - Provider product page
- Provider
- OriGene
- Product name
- PIM1 Antibody (C-term ) Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- PIM1 Antibody (C-term ) Rabbit Polyclonal Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PIM1
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PIM1 Pab (Cat. #TA300039) in A549 cell lysate. PIM1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PIM1 (arrow) using PIM1 Antibody (C-term) (Cat.#TA300039). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC
