PA5-28231

antibody from Invitrogen Antibodies

Targeting: ACVR2B ActR-IIB

Antibody data

Submit

Validation data

Product number: PA5-28231 - Provider product page
Provider: Invitrogen Antibodies
Product name: ACVR2B Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: U87-MG, SK-N-SH, IMR-32, SK-N-AS, mosue cerebellum, mouse testis, ACVR2B-transfected 293T. Predicted reactivity: Mouse (99%), Rat (99%), Pig (100%), Chicken (89%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.45 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ACVR2B protein structure - PA5-28231 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Activin Receptor Type IIB using 30 µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Activin Receptor Type IIB polyclonal antibody (Product # PA5-28231) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ACVR2B was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ACVR2B Polyclonal Antibody (Product # PA5-28231) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ACVR2B was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ACVR2B Polyclonal Antibody (Product # PA5-28231) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ACVR2B was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ACVR2B Polyclonal Antibody (Product # PA5-28231) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.