PA5-28231

antibody from Invitrogen Antibodies

Targeting: ACVR2B ActR-IIB

Antibody data

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Validation data

Product number: PA5-28231 - Provider product page
Provider: Invitrogen Antibodies
Product name: ACVR2B Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: U87-MG, SK-N-SH, IMR-32, SK-N-AS, mosue cerebellum, mouse testis, ACVR2B-transfected 293T.
Concentration: 0.91 mg/mL

ACVR2B protein structure - PA5-28231 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Activin Receptor Type IIB using 30 µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Activin Receptor Type IIB polyclonal antibody (Product # PA5-28231) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ACVR2B was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ACVR2B Polyclonal Antibody (Product # PA5-28231) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ACVR2B was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ACVR2B Polyclonal Antibody (Product # PA5-28231) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ACVR2B was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ACVR2B Polyclonal Antibody (Product # PA5-28231) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.