MA5-60774

antibody from Invitrogen Antibodies

Targeting: ACVR2B ActR-IIB

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MA5-60774

Provider

Invitrogen Antibodies

Product name

ACVR2B Recombinant Human Monoclonal Antibody (7E5)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Human

Isotype

IgG

Antibody clone number

7.00E+05

Vial size

100 µL

Concentration

3 mg/mL

Storage

-20°C or -80°C if preferred

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of ACVR2B. Samples were incubated with ACVR2B Recombinant Monoclonal antibody (Product # MA5-60774) using a dilution of 2 µg/mL. Activity: Measured by its binding ability in a functional ELISA. The EC50 is 3.560-4.235 ng/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of ACVR2B. Samples were incubated with ACVR2B Recombinant Monoclonal antibody (Product # MA5-60774) using a dilution of 2 µg/mL. Activity: Measured by its binding ability in a functional ELISA. The EC50 is 1.562-1.966 ng/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of ACVR2B. Samples were incubated with ACVR2B Recombinant Monoclonal antibody (Product # MA5-60774) using a dilution of 2 µg/mL. Activity: Measured by its binding ability in a functional ELISA. The EC50 is 2.983-3.438 ng/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of ACVR2B. Samples were incubated with ACVR2B Recombinant Monoclonal antibody (Product # MA5-60774) using a dilution of 2 µg/mL. Activity: Measured by its binding ability in a functional ELISA. The EC50 is 3.848-4.375 ng/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ACVR2B in SH-SY5Y cells. Samples were incubated with ACVR2B Recombinant Monoclonal antibody (Product # MA5-60774) using a dilution of 1:100 followed by FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.